Defense for the title Dr. rer. nat.

I had four examiners of different field: parasitology, biochemistry, genetics and veterinary physiology (all male).

Hints for your persentation

• Presentation time: 20-30 min; the best will be 25-30 min but never exceed 30 min
• Avoid saying ‘we did’ ‘we have’; instead it should be ‘I did’ because this is your work
• Talk as clearly as possible

Sum of questions

Parasitology

• advantage of schistosomes over other hermaphodatic trematode (higher efficiency in reproduction)
• RNA-Seq compared to other transcriptome approaches i.e. microarray, SAGE
• function of hypothetical proteins in the gonads if they are gonad-enriched
• comparison to other gonad transcriptome studies

Vet Physiology

• capacitative Ca2+ entry (store-mediated)
• what are the vitelline cells used for

Genetics

• autosomes, sex chromosome
• genome size and number of genes (human, schisto)
• alternative splicing (ratio, examples)
• rRNA, ribosome
• proteome possibility

Biochemistry

• origin of autofluorescence
• metabolic pathways in the vitelline cells
• RNA-Seq technology
• stored mRNA
• data validation methods (qPCR)
• ways for gene function prediction
• CPEB function
• phosphotase and kinase

PhD is not a final end. Keep learning!